For the immunological determination of luteinizing hormone(L. H.) in the urine of adult men, basic investigation and semi-quantitative assay has been performed using haemagglutination inhibition reaction based upon the immunologic cross-reaction between the H. C. G.antiserum and L. H. Following results were obtained.
1) Human Chorionic Gonadotropin(H. C. G.) of relatively high purity (5, 0001. U. C mg) have been used as antigen for the immunization of rabbits and high titer of antiserum was obtained through the effects of Freund´s adjuvant and booster injections.
2) Gel diffusion method, of Ouchterlony revealed only one clear precipitation line between the H. C. G. and its antiserum, indicating that the used antigen was relatively pure and the antisera contained negligible amount of non-specific antibody.
3) The sensitivity of haemagglutination reaction in this study was about 251. U. /L.
4) Early morning samples of urine were collected from 27 adult men and 6 infertile men.
5) One volume of urine was treated with four volumes of acetone and L. H. containing fraction was washed with one volume of absolute alcohol and ether, then dissolved in 1/10 volume of pH 6.4, phosphate buffered saline, thus, concentrating the original specimen 10 times.
6) The concentration of L. H. in the morning urine of adult men and infertile men. varied. between 25-120 i. U. /L and 30-100 I. U. /L respectiveely. It revealed that
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